A safe and convenient pseudovirus-based inhibition assay to detect neutralizing antibodies and screen for viral entry inhibitors against the novel human coronavirus MERS-CoV
Identifieur interne : 002064 ( Main/Exploration ); précédent : 002063; suivant : 002065A safe and convenient pseudovirus-based inhibition assay to detect neutralizing antibodies and screen for viral entry inhibitors against the novel human coronavirus MERS-CoV
Auteurs : Guangyu Zhao [République populaire de Chine] ; Lanying Du [États-Unis] ; Cuiqing Ma [États-Unis] ; Ye Li [États-Unis] ; Lin Li [République populaire de Chine] ; Vincent Km Poon [République populaire de Chine] ; Lili Wang [États-Unis] ; Fei Yu [États-Unis] ; Bo-Jian Zheng [République populaire de Chine] ; Shibo Jiang [États-Unis, République populaire de Chine] ; Yusen Zhou [République populaire de Chine]Source :
- Virology Journal [ 1743-422X ] ; 2013.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (isolement et purification), Anticorps neutralisants (isolement et purification), Antiviraux (isolement et purification), Coloration et marquage (), Coronavirus (), Coronavirus (immunologie), Glycoprotéine de spicule des coronavirus (génétique), Glycoprotéine de spicule des coronavirus (métabolisme), Humains, Lignée cellulaire, Luciferases (analyse), Luciferases (génétique), Souris, Tests de neutralisation (), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique), Évaluation préclinique de médicament ().
- MESH :
- analyse : Luciferases.
- génétique : Glycoprotéine de spicule des coronavirus, Luciferases, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- immunologie : Coronavirus.
- isolement et purification : Anticorps antiviraux, Anticorps neutralisants, Antiviraux.
- métabolisme : Glycoprotéine de spicule des coronavirus.
- Animaux, Coloration et marquage, Coronavirus, Humains, Lignée cellulaire, Souris, Tests de neutralisation, Évaluation préclinique de médicament.
English descriptors
- KwdEn :
- Animals, Antibodies, Neutralizing (isolation & purification), Antibodies, Viral (isolation & purification), Antiviral Agents (isolation & purification), Cell Line, Coronavirus (drug effects), Coronavirus (immunology), Drug Evaluation, Preclinical (methods), HIV-1 (genetics), Humans, Luciferases (analysis), Luciferases (genetics), Mice, Neutralization Tests (methods), Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (metabolism), Staining and Labeling (methods).
- MESH :
- chemical , analysis : Luciferases.
- chemical , genetics : Luciferases, Spike Glycoprotein, Coronavirus.
- chemical , isolation & purification : Antibodies, Neutralizing, Antibodies, Viral, Antiviral Agents.
- drug effects : Coronavirus.
- genetics : HIV-1.
- immunology : Coronavirus.
- chemical , metabolism : Spike Glycoprotein, Coronavirus.
- methods : Drug Evaluation, Preclinical, Neutralization Tests, Staining and Labeling.
- Animals, Cell Line, Humans, Mice.
Abstract
Evidence points to the emergence of a novel human coronavirus, Middle East respiratory syndrome coronavirus (MERS-CoV), which causes a severe acute respiratory syndrome (SARS)-like disease. In response, the development of effective vaccines and therapeutics remains a clinical priority. To accomplish this, it is necessary to evaluate neutralizing antibodies and screen for MERS-CoV entry inhibitors.
In this study, we produced a pseudovirus bearing the full-length spike (S) protein of MERS-CoV in the Env-defective, luciferase-expressing HIV-1 backbone. We then established a pseudovirus-based inhibition assay to detect neutralizing antibodies and anti-MERS-CoV entry inhibitors.
Our results demonstrated that the generated MERS-CoV pseudovirus allows for single-cycle infection of a variety of cells expressing dipeptidyl peptidase-4 (DPP4), the confirmed receptor for MERS-CoV. Consistent with the results from a live MERS-CoV-based inhibition assay, the antisera of mice vaccinated with a recombinant protein containing receptor-binding domain (RBD, residues 377–662) of MERS-CoV S fused with Fc of human IgG exhibited neutralizing antibody response against infection of MERS-CoV pseudovirus. Furthermore, one small molecule HIV entry inhibitor targeting gp41 (ADS-J1) and the 3-hydroxyphthalic anhydride-modified human serum albumin (HP-HSA) could significantly inhibit MERS-CoV pseudovirus infection.
Taken together, the established MERS-CoV inhibition assay is a safe and convenient pseudovirus-based alternative to BSL-3 live-virus restrictions and can be used to rapidly screen MERS-CoV entry inhibitors, as well as evaluate vaccine-induced neutralizing antibodies against the highly pathogenic MERS-CoV.
The online version of this article (doi:10.1186/1743-422X-10-266) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/1743-422X-10-266
PubMed: 23978242
PubMed Central: 3765664
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000473
- to stream Pmc, to step Curation: 000473
- to stream Pmc, to step Checkpoint: 001267
- to stream PubMed, to step Corpus: 001C07
- to stream PubMed, to step Curation: 001C07
- to stream PubMed, to step Checkpoint: 001C12
- to stream Ncbi, to step Merge: 000B31
- to stream Ncbi, to step Curation: 000B31
- to stream Ncbi, to step Checkpoint: 000B31
- to stream Main, to step Merge: 002088
- to stream Main, to step Curation: 002064
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A safe and convenient pseudovirus-based inhibition assay to detect neutralizing antibodies and screen for viral entry inhibitors against the novel human coronavirus MERS-CoV</title><author><name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>State Key Laboratory of Pathogen and Biosecurity,</institution><institution>Beijing Institute of Microbiology and Epidemiology,</institution></institution-wrap>Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name><affiliation wicri:level="2"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Lindsley F. Kimball Research Institute,New York Blood Center,</institution></institution-wrap>New York, NY USA</nlm:aff><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>New York</wicri:cityArea></affiliation></author><author><name sortKey="Ma, Cuiqing" sort="Ma, Cuiqing" uniqKey="Ma C" first="Cuiqing" last="Ma">Cuiqing Ma</name><affiliation wicri:level="2"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Lindsley F. Kimball Research Institute,New York Blood Center,</institution></institution-wrap>New York, NY USA</nlm:aff><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>New York</wicri:cityArea></affiliation></author><author><name sortKey="Li, Ye" sort="Li, Ye" uniqKey="Li Y" first="Ye" last="Li">Ye Li</name><affiliation wicri:level="2"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Lindsley F. Kimball Research Institute,New York Blood Center,</institution></institution-wrap>New York, NY USA</nlm:aff><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>New York</wicri:cityArea></affiliation></author><author><name sortKey="Li, Lin" sort="Li, Lin" uniqKey="Li L" first="Lin" last="Li">Lin Li</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>State Key Laboratory of Pathogen and Biosecurity,</institution><institution>Beijing Institute of Microbiology and Epidemiology,</institution></institution-wrap>Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Poon, Vincent Km" sort="Poon, Vincent Km" uniqKey="Poon V" first="Vincent Km" last="Poon">Vincent Km Poon</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.250415.7</institution-id><institution-id institution-id-type="ISNI">0000000404422075</institution-id><institution>Department of Microbiology,</institution><institution>the University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Lili" sort="Wang, Lili" uniqKey="Wang L" first="Lili" last="Wang">Lili Wang</name><affiliation wicri:level="2"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Lindsley F. Kimball Research Institute,New York Blood Center,</institution></institution-wrap>New York, NY USA</nlm:aff><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>New York</wicri:cityArea></affiliation></author><author><name sortKey="Yu, Fei" sort="Yu, Fei" uniqKey="Yu F" first="Fei" last="Yu">Fei Yu</name><affiliation wicri:level="2"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Lindsley F. Kimball Research Institute,New York Blood Center,</institution></institution-wrap>New York, NY USA</nlm:aff><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>New York</wicri:cityArea></affiliation></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.250415.7</institution-id><institution-id institution-id-type="ISNI">0000000404422075</institution-id><institution>Department of Microbiology,</institution><institution>the University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><affiliation wicri:level="2"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Lindsley F. Kimball Research Institute,New York Blood Center,</institution></institution-wrap>New York, NY USA</nlm:aff><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>New York</wicri:cityArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College and Institute of Medical Microbiology,</institution><institution>Fudan University,</institution></institution-wrap>Shanghai, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>State Key Laboratory of Pathogen and Biosecurity,</institution><institution>Beijing Institute of Microbiology and Epidemiology,</institution></institution-wrap>Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23978242</idno><idno type="pmc">3765664</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765664</idno><idno type="RBID">PMC:3765664</idno><idno type="doi">10.1186/1743-422X-10-266</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000473</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000473</idno><idno type="wicri:Area/Pmc/Curation">000473</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000473</idno><idno type="wicri:Area/Pmc/Checkpoint">001267</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001267</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:23978242</idno><idno type="wicri:Area/PubMed/Corpus">001C07</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001C07</idno><idno type="wicri:Area/PubMed/Curation">001C07</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001C07</idno><idno type="wicri:Area/PubMed/Checkpoint">001C12</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001C12</idno><idno type="wicri:Area/Ncbi/Merge">000B31</idno><idno type="wicri:Area/Ncbi/Curation">000B31</idno><idno type="wicri:Area/Ncbi/Checkpoint">000B31</idno><idno type="wicri:Area/Main/Merge">002088</idno><idno type="wicri:Area/Main/Curation">002064</idno><idno type="wicri:Area/Main/Exploration">002064</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A safe and convenient pseudovirus-based inhibition assay to detect neutralizing antibodies and screen for viral entry inhibitors against the novel human coronavirus MERS-CoV</title><author><name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>State Key Laboratory of Pathogen and Biosecurity,</institution><institution>Beijing Institute of Microbiology and Epidemiology,</institution></institution-wrap>Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name><affiliation wicri:level="2"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Lindsley F. Kimball Research Institute,New York Blood Center,</institution></institution-wrap>New York, NY USA</nlm:aff><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>New York</wicri:cityArea></affiliation></author><author><name sortKey="Ma, Cuiqing" sort="Ma, Cuiqing" uniqKey="Ma C" first="Cuiqing" last="Ma">Cuiqing Ma</name><affiliation wicri:level="2"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Lindsley F. Kimball Research Institute,New York Blood Center,</institution></institution-wrap>New York, NY USA</nlm:aff><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>New York</wicri:cityArea></affiliation></author><author><name sortKey="Li, Ye" sort="Li, Ye" uniqKey="Li Y" first="Ye" last="Li">Ye Li</name><affiliation wicri:level="2"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Lindsley F. Kimball Research Institute,New York Blood Center,</institution></institution-wrap>New York, NY USA</nlm:aff><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>New York</wicri:cityArea></affiliation></author><author><name sortKey="Li, Lin" sort="Li, Lin" uniqKey="Li L" first="Lin" last="Li">Lin Li</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>State Key Laboratory of Pathogen and Biosecurity,</institution><institution>Beijing Institute of Microbiology and Epidemiology,</institution></institution-wrap>Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Poon, Vincent Km" sort="Poon, Vincent Km" uniqKey="Poon V" first="Vincent Km" last="Poon">Vincent Km Poon</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.250415.7</institution-id><institution-id institution-id-type="ISNI">0000000404422075</institution-id><institution>Department of Microbiology,</institution><institution>the University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Lili" sort="Wang, Lili" uniqKey="Wang L" first="Lili" last="Wang">Lili Wang</name><affiliation wicri:level="2"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Lindsley F. Kimball Research Institute,New York Blood Center,</institution></institution-wrap>New York, NY USA</nlm:aff><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>New York</wicri:cityArea></affiliation></author><author><name sortKey="Yu, Fei" sort="Yu, Fei" uniqKey="Yu F" first="Fei" last="Yu">Fei Yu</name><affiliation wicri:level="2"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Lindsley F. Kimball Research Institute,New York Blood Center,</institution></institution-wrap>New York, NY USA</nlm:aff><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>New York</wicri:cityArea></affiliation></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.250415.7</institution-id><institution-id institution-id-type="ISNI">0000000404422075</institution-id><institution>Department of Microbiology,</institution><institution>the University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><affiliation wicri:level="2"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Lindsley F. Kimball Research Institute,New York Blood Center,</institution></institution-wrap>New York, NY USA</nlm:aff><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>New York</wicri:cityArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College and Institute of Medical Microbiology,</institution><institution>Fudan University,</institution></institution-wrap>Shanghai, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>State Key Laboratory of Pathogen and Biosecurity,</institution><institution>Beijing Institute of Microbiology and Epidemiology,</institution></institution-wrap>Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author></analytic><series><title level="j">Virology Journal</title><idno type="eISSN">1743-422X</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Neutralizing (isolation & purification)</term><term>Antibodies, Viral (isolation & purification)</term><term>Antiviral Agents (isolation & purification)</term><term>Cell Line</term><term>Coronavirus (drug effects)</term><term>Coronavirus (immunology)</term><term>Drug Evaluation, Preclinical (methods)</term><term>HIV-1 (genetics)</term><term>Humans</term><term>Luciferases (analysis)</term><term>Luciferases (genetics)</term><term>Mice</term><term>Neutralization Tests (methods)</term><term>Spike Glycoprotein, Coronavirus (genetics)</term><term>Spike Glycoprotein, Coronavirus (metabolism)</term><term>Staining and Labeling (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (isolement et purification)</term><term>Anticorps neutralisants (isolement et purification)</term><term>Antiviraux (isolement et purification)</term><term>Coloration et marquage ()</term><term>Coronavirus ()</term><term>Coronavirus (immunologie)</term><term>Glycoprotéine de spicule des coronavirus (génétique)</term><term>Glycoprotéine de spicule des coronavirus (métabolisme)</term><term>Humains</term><term>Lignée cellulaire</term><term>Luciferases (analyse)</term><term>Luciferases (génétique)</term><term>Souris</term><term>Tests de neutralisation ()</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique)</term><term>Évaluation préclinique de médicament ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Luciferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Luciferases</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term><term>Antiviral Agents</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Luciferases</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term><term>Luciferases</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps neutralisants</term><term>Antiviraux</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Drug Evaluation, Preclinical</term><term>Neutralization Tests</term><term>Staining and Labeling</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Humans</term><term>Mice</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Coloration et marquage</term><term>Coronavirus</term><term>Humains</term><term>Lignée cellulaire</term><term>Souris</term><term>Tests de neutralisation</term><term>Évaluation préclinique de médicament</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Evidence points to the emergence of a novel human coronavirus, Middle East respiratory syndrome coronavirus (MERS-CoV), which causes a severe acute respiratory syndrome (SARS)-like disease. In response, the development of effective vaccines and therapeutics remains a clinical priority. To accomplish this, it is necessary to evaluate neutralizing antibodies and screen for MERS-CoV entry inhibitors.</p></sec><sec><title>Methods</title><p>In this study, we produced a pseudovirus bearing the full-length spike (S) protein of MERS-CoV in the Env-defective, luciferase-expressing HIV-1 backbone. We then established a pseudovirus-based inhibition assay to detect neutralizing antibodies and anti-MERS-CoV entry inhibitors.</p></sec><sec><title>Results</title><p>Our results demonstrated that the generated MERS-CoV pseudovirus allows for single-cycle infection of a variety of cells expressing dipeptidyl peptidase-4 (DPP4), the confirmed receptor for MERS-CoV. Consistent with the results from a live MERS-CoV-based inhibition assay, the antisera of mice vaccinated with a recombinant protein containing receptor-binding domain (RBD, residues 377–662) of MERS-CoV S fused with Fc of human IgG exhibited neutralizing antibody response against infection of MERS-CoV pseudovirus. Furthermore, one small molecule HIV entry inhibitor targeting gp41 (ADS-J1) and the 3-hydroxyphthalic anhydride-modified human serum albumin (HP-HSA) could significantly inhibit MERS-CoV pseudovirus infection.</p></sec><sec><title>Conclusion</title><p>Taken together, the established MERS-CoV inhibition assay is a safe and convenient pseudovirus-based alternative to BSL-3 live-virus restrictions and can be used to rapidly screen MERS-CoV entry inhibitors, as well as evaluate vaccine-induced neutralizing antibodies against the highly pathogenic MERS-CoV.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1743-422X-10-266) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chan, Jf" uniqKey="Chan J">JF Chan</name></author><author><name sortKey="Li, Ks" uniqKey="Li K">KS Li</name></author><author><name sortKey="To, Kk" uniqKey="To K">KK To</name></author><author><name sortKey="Cheng, Vc" uniqKey="Cheng V">VC Cheng</name></author><author><name sortKey="Chen, H" uniqKey="Chen H">H Chen</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van, Bs" uniqKey="Van B">BS Van</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pollack, Mp" uniqKey="Pollack M">MP Pollack</name></author><author><name sortKey="Pringle, C" uniqKey="Pringle C">C Pringle</name></author><author><name sortKey="Madoff, Lc" uniqKey="Madoff L">LC Madoff</name></author><author><name sortKey="Memish, Za" uniqKey="Memish Z">ZA Memish</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Memish, Za" uniqKey="Memish Z">ZA Memish</name></author><author><name sortKey="Zumla, Ai" uniqKey="Zumla A">AI Zumla</name></author><author><name sortKey="Al Hakeem, Rf" uniqKey="Al Hakeem R">RF Al-Hakeem</name></author><author><name sortKey="Al Rabeeah, Aa" uniqKey="Al Rabeeah A">AA Al-Rabeeah</name></author><author><name sortKey="Stephens, Gm" uniqKey="Stephens G">GM Stephens</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author><author><name sortKey="He, Y" uniqKey="He Y">Y He</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y Zhou</name></author><author><name sortKey="Liu, S" uniqKey="Liu S">S Liu</name></author><author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van, Bs" uniqKey="Van B">BS Van</name></author><author><name sortKey="De, Gm" uniqKey="De G">GM De</name></author><author><name sortKey="Lauber, C" uniqKey="Lauber C">C Lauber</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Raj, Vs" uniqKey="Raj V">VS Raj</name></author><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Haagmans, Bl" uniqKey="Haagmans B">BL Haagmans</name></author><author><name sortKey="Gorbalenya, Ae" uniqKey="Gorbalenya A">AE Gorbalenya</name></author><author><name sortKey="Snijder, Ej" uniqKey="Snijder E">EJ Snijder</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author><author><name sortKey="Moore, Mj" uniqKey="Moore M">MJ Moore</name></author><author><name sortKey="Vasilieva, N" uniqKey="Vasilieva N">N Vasilieva</name></author><author><name sortKey="Sui, J" uniqKey="Sui J">J Sui</name></author><author><name sortKey="Wong, Sk" uniqKey="Wong S">SK Wong</name></author><author><name sortKey="Berne, Ma" uniqKey="Berne M">MA Berne</name></author><author><name sortKey="Somasundaran, M" uniqKey="Somasundaran M">M Somasundaran</name></author><author><name sortKey="Sullivan, Jl" uniqKey="Sullivan J">JL Sullivan</name></author><author><name sortKey="Luzuriaga, K" uniqKey="Luzuriaga K">K Luzuriaga</name></author><author><name sortKey="Greenough, Tc" uniqKey="Greenough T">TC Greenough</name></author><author><name sortKey="Choe, H" uniqKey="Choe H">H Choe</name></author><author><name sortKey="Farzan, M" uniqKey="Farzan M">M Farzan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Raj, Vs" uniqKey="Raj V">VS Raj</name></author><author><name sortKey="Mou, H" uniqKey="Mou H">H Mou</name></author><author><name sortKey="Smits, Sl" uniqKey="Smits S">SL Smits</name></author><author><name sortKey="Dekkers, Dh" uniqKey="Dekkers D">DH Dekkers</name></author><author><name sortKey="Muller, Ma" uniqKey="Muller M">MA Muller</name></author><author><name sortKey="Dijkman, R" uniqKey="Dijkman R">R Dijkman</name></author><author><name sortKey="Muth, D" uniqKey="Muth D">D Muth</name></author><author><name sortKey="Demmers, Ja" uniqKey="Demmers J">JA Demmers</name></author><author><name sortKey="Zaki, A" uniqKey="Zaki A">A Zaki</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author><author><name sortKey="Thiel, V" uniqKey="Thiel V">V Thiel</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Rottier, Pj" uniqKey="Rottier P">PJ Rottier</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Bosch, Bj" uniqKey="Bosch B">BJ Bosch</name></author><author><name sortKey="Haagmans, Bl" uniqKey="Haagmans B">BL Haagmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gierer, S" uniqKey="Gierer S">S Gierer</name></author><author><name sortKey="Bertram, S" uniqKey="Bertram S">S Bertram</name></author><author><name sortKey="Kaup, F" uniqKey="Kaup F">F Kaup</name></author><author><name sortKey="Wrensch, F" uniqKey="Wrensch F">F Wrensch</name></author><author><name sortKey="Heurich, A" uniqKey="Heurich A">A Heurich</name></author><author><name sortKey="Kramer Kuhl, A" uniqKey="Kramer Kuhl A">A Kramer-Kuhl</name></author><author><name sortKey="Welsch, K" uniqKey="Welsch K">K Welsch</name></author><author><name sortKey="Winkler, M" uniqKey="Winkler M">M Winkler</name></author><author><name sortKey="Meyer, B" uniqKey="Meyer B">B Meyer</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Dittmer, U" uniqKey="Dittmer U">U Dittmer</name></author><author><name sortKey="Von, Ht" uniqKey="Von H">HT Von</name></author><author><name sortKey="Simmons, G" uniqKey="Simmons G">G Simmons</name></author><author><name sortKey="Hofmann, H" uniqKey="Hofmann H">H Hofmann</name></author><author><name sortKey="Pohlmann, S" uniqKey="Pohlmann S">S Pohlmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author><author><name sortKey="Zhao, G" uniqKey="Zhao G">G Zhao</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X Zhang</name></author><author><name sortKey="Liu, Z" uniqKey="Liu Z">Z Liu</name></author><author><name sortKey="Yu, H" uniqKey="Yu H">H Yu</name></author><author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y Zhou</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author><author><name sortKey="Zhao, G" uniqKey="Zhao G">G Zhao</name></author><author><name sortKey="Kou, Z" uniqKey="Kou Z">Z Kou</name></author><author><name sortKey="Ma, C" uniqKey="Ma C">C Ma</name></author><author><name sortKey="Sun, S" uniqKey="Sun S">S Sun</name></author><author><name sortKey="Poon, Vk" uniqKey="Poon V">VK Poon</name></author><author><name sortKey="Lu, L" uniqKey="Lu L">L Lu</name></author><author><name sortKey="Wang, L" uniqKey="Wang L">L Wang</name></author><author><name sortKey="Debnath, Ak" uniqKey="Debnath A">AK Debnath</name></author><author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y Zhou</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Debnath, Ak" uniqKey="Debnath A">AK Debnath</name></author><author><name sortKey="Radigan, L" uniqKey="Radigan L">L Radigan</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, H" uniqKey="Wang H">H Wang</name></author><author><name sortKey="Qi, Z" uniqKey="Qi Z">Z Qi</name></author><author><name sortKey="Guo, A" uniqKey="Guo A">A Guo</name></author><author><name sortKey="Mao, Q" uniqKey="Mao Q">Q Mao</name></author><author><name sortKey="Lu, H" uniqKey="Lu H">H Lu</name></author><author><name sortKey="An, X" uniqKey="An X">X An</name></author><author><name sortKey="Xia, C" uniqKey="Xia C">C Xia</name></author><author><name sortKey="Li, X" uniqKey="Li X">X Li</name></author><author><name sortKey="Debnath, Ak" uniqKey="Debnath A">AK Debnath</name></author><author><name sortKey="Wu, S" uniqKey="Wu S">S Wu</name></author><author><name sortKey="Liu, S" uniqKey="Liu S">S Liu</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, L" uniqKey="Li L">L Li</name></author><author><name sortKey="Qiu, J" uniqKey="Qiu J">J Qiu</name></author><author><name sortKey="Lu, L" uniqKey="Lu L">L Lu</name></author><author><name sortKey="An, S" uniqKey="An S">S An</name></author><author><name sortKey="Qiao, P" uniqKey="Qiao P">P Qiao</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author><author><name sortKey="Liu, S" uniqKey="Liu S">S Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, S" uniqKey="Liu S">S Liu</name></author><author><name sortKey="Jing, W" uniqKey="Jing W">W Jing</name></author><author><name sortKey="Cheung, B" uniqKey="Cheung B">B Cheung</name></author><author><name sortKey="Lu, H" uniqKey="Lu H">H Lu</name></author><author><name sortKey="Sun, J" uniqKey="Sun J">J Sun</name></author><author><name sortKey="Yan, X" uniqKey="Yan X">X Yan</name></author><author><name sortKey="Niu, J" uniqKey="Niu J">J Niu</name></author><author><name sortKey="Farmar, J" uniqKey="Farmar J">J Farmar</name></author><author><name sortKey="Wu, S" uniqKey="Wu S">S Wu</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chu, Lh" uniqKey="Chu L">LH Chu</name></author><author><name sortKey="Chan, Sh" uniqKey="Chan S">SH Chan</name></author><author><name sortKey="Tsai, Sn" uniqKey="Tsai S">SN Tsai</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Cheng, Ch" uniqKey="Cheng C">CH Cheng</name></author><author><name sortKey="Wong, Kb" uniqKey="Wong K">KB Wong</name></author><author><name sortKey="Waye, Mm" uniqKey="Waye M">MM Waye</name></author><author><name sortKey="Ngai, Sm" uniqKey="Ngai S">SM Ngai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, L" uniqKey="Lu L">L Lu</name></author><author><name sortKey="Yang, X" uniqKey="Yang X">X Yang</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fuk Woo, Cj" uniqKey="Fuk Woo C">CJ Fuk-Woo</name></author><author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name></author><author><name sortKey="Choi, Gk" uniqKey="Choi G">GK Choi</name></author><author><name sortKey="To, Kk" uniqKey="To K">KK To</name></author><author><name sortKey="Tse, H" uniqKey="Tse H">H Tse</name></author><author><name sortKey="Cai, Jp" uniqKey="Cai J">JP Cai</name></author><author><name sortKey="Yeung, Ml" uniqKey="Yeung M">ML Yeung</name></author><author><name sortKey="Cheng, Vc" uniqKey="Cheng V">VC Cheng</name></author><author><name sortKey="Chen, H" uniqKey="Chen H">H Chen</name></author><author><name sortKey="Che, Xy" uniqKey="Che X">XY Che</name></author><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jeffers, Sa" uniqKey="Jeffers S">SA Jeffers</name></author><author><name sortKey="Tusell, Sm" uniqKey="Tusell S">SM Tusell</name></author><author><name sortKey="Gillim Ross, L" uniqKey="Gillim Ross L">L Gillim-Ross</name></author><author><name sortKey="Hemmila, Em" uniqKey="Hemmila E">EM Hemmila</name></author><author><name sortKey="Achenbach, Je" uniqKey="Achenbach J">JE Achenbach</name></author><author><name sortKey="Babcock, Gj" uniqKey="Babcock G">GJ Babcock</name></author><author><name sortKey="Thomas, Wd" uniqKey="Thomas W">WD Thomas</name></author><author><name sortKey="Thackray, Lb" uniqKey="Thackray L">LB Thackray</name></author><author><name sortKey="Young, Md" uniqKey="Young M">MD Young</name></author><author><name sortKey="Mason, Rj" uniqKey="Mason R">RJ Mason</name></author><author><name sortKey="Ambrosino, Dm" uniqKey="Ambrosino D">DM Ambrosino</name></author><author><name sortKey="Wentworth, De" uniqKey="Wentworth D">DE Wentworth</name></author><author><name sortKey="Demartini, Jc" uniqKey="Demartini J">JC Demartini</name></author><author><name sortKey="Holmes, Kv" uniqKey="Holmes K">KV Holmes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Zy" uniqKey="Yang Z">ZY Yang</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Ganesh, L" uniqKey="Ganesh L">L Ganesh</name></author><author><name sortKey="Leung, K" uniqKey="Leung K">K Leung</name></author><author><name sortKey="Kong, Wp" uniqKey="Kong W">WP Kong</name></author><author><name sortKey="Schwartz, O" uniqKey="Schwartz O">O Schwartz</name></author><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K Subbarao</name></author><author><name sortKey="Nabel, Gj" uniqKey="Nabel G">GJ Nabel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Faber, M" uniqKey="Faber M">M Faber</name></author><author><name sortKey="Lamirande, Ew" uniqKey="Lamirande E">EW Lamirande</name></author><author><name sortKey="Roberts, A" uniqKey="Roberts A">A Roberts</name></author><author><name sortKey="Rice, Ab" uniqKey="Rice A">AB Rice</name></author><author><name sortKey="Koprowski, H" uniqKey="Koprowski H">H Koprowski</name></author><author><name sortKey="Dietzschold, B" uniqKey="Dietzschold B">B Dietzschold</name></author><author><name sortKey="Schnell, Mj" uniqKey="Schnell M">MJ Schnell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pang, H" uniqKey="Pang H">H Pang</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name></author><author><name sortKey="Han, X" uniqKey="Han X">X Han</name></author><author><name sortKey="Xu, Y" uniqKey="Xu Y">Y Xu</name></author><author><name sortKey="Jiang, F" uniqKey="Jiang F">F Jiang</name></author><author><name sortKey="Wu, D" uniqKey="Wu D">D Wu</name></author><author><name sortKey="Kong, X" uniqKey="Kong X">X Kong</name></author><author><name sortKey="Bartlam, M" uniqKey="Bartlam M">M Bartlam</name></author><author><name sortKey="Rao, Z" uniqKey="Rao Z">Z Rao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Temperton, Nj" uniqKey="Temperton N">NJ Temperton</name></author><author><name sortKey="Chan, Pk" uniqKey="Chan P">PK Chan</name></author><author><name sortKey="Simmons, G" uniqKey="Simmons G">G Simmons</name></author><author><name sortKey="Zambon, Mc" uniqKey="Zambon M">MC Zambon</name></author><author><name sortKey="Tedder, Rs" uniqKey="Tedder R">RS Tedder</name></author><author><name sortKey="Takeuchi, Y" uniqKey="Takeuchi Y">Y Takeuchi</name></author><author><name sortKey="Weiss, Ra" uniqKey="Weiss R">RA Weiss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mou, H" uniqKey="Mou H">H Mou</name></author><author><name sortKey="Raj, Vs" uniqKey="Raj V">VS Raj</name></author><author><name sortKey="Van Kuppeveld, Fj" uniqKey="Van Kuppeveld F">FJ Van Kuppeveld</name></author><author><name sortKey="Rottier, Pj" uniqKey="Rottier P">PJ Rottier</name></author><author><name sortKey="Haagmans, Bl" uniqKey="Haagmans B">BL Haagmans</name></author><author><name sortKey="Bosch, Bj" uniqKey="Bosch B">BJ Bosch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author><author><name sortKey="Zhao, G" uniqKey="Zhao G">G Zhao</name></author><author><name sortKey="Sun, S" uniqKey="Sun S">S Sun</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X Zhang</name></author><author><name sortKey="Zhou, X" uniqKey="Zhou X">X Zhou</name></author><author><name sortKey="Guo, Y" uniqKey="Guo Y">Y Guo</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y Zhou</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author><author><name sortKey="Zhao, G" uniqKey="Zhao G">G Zhao</name></author><author><name sortKey="Li, L" uniqKey="Li L">L Li</name></author><author><name sortKey="He, Y" uniqKey="He Y">Y He</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y Zhou</name></author><author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author><author><name sortKey="Zhao, G" uniqKey="Zhao G">G Zhao</name></author><author><name sortKey="Lin, Y" uniqKey="Lin Y">Y Lin</name></author><author><name sortKey="Sui, H" uniqKey="Sui H">H Sui</name></author><author><name sortKey="Chan, C" uniqKey="Chan C">C Chan</name></author><author><name sortKey="Ma, S" uniqKey="Ma S">S Ma</name></author><author><name sortKey="He, Y" uniqKey="He Y">Y He</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author><author><name sortKey="Wu, C" uniqKey="Wu C">C Wu</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author><author><name sortKey="Jin, Dy" uniqKey="Jin D">DY Jin</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y Zhou</name></author><author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author><author><name sortKey="Zhao, G" uniqKey="Zhao G">G Zhao</name></author><author><name sortKey="Chan, Cc" uniqKey="Chan C">CC Chan</name></author><author><name sortKey="Sun, S" uniqKey="Sun S">S Sun</name></author><author><name sortKey="Chen, M" uniqKey="Chen M">M Chen</name></author><author><name sortKey="Liu, Z" uniqKey="Liu Z">Z Liu</name></author><author><name sortKey="Guo, H" uniqKey="Guo H">H Guo</name></author><author><name sortKey="He, Y" uniqKey="He Y">Y He</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y Zhou</name></author><author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, L" uniqKey="Li L">L Li</name></author><author><name sortKey="Qiao, P" uniqKey="Qiao P">P Qiao</name></author><author><name sortKey="Yang, J" uniqKey="Yang J">J Yang</name></author><author><name sortKey="Lu, L" uniqKey="Lu L">L Lu</name></author><author><name sortKey="Tan, S" uniqKey="Tan S">S Tan</name></author><author><name sortKey="Lu, H" uniqKey="Lu H">H Lu</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X Zhang</name></author><author><name sortKey="Chen, X" uniqKey="Chen X">X Chen</name></author><author><name sortKey="Wu, S" uniqKey="Wu S">S Wu</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author><author><name sortKey="Liu, S" uniqKey="Liu S">S Liu</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name></noRegion><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><name sortKey="Li, Lin" sort="Li, Lin" uniqKey="Li L" first="Lin" last="Li">Lin Li</name><name sortKey="Poon, Vincent Km" sort="Poon, Vincent Km" uniqKey="Poon V" first="Vincent Km" last="Poon">Vincent Km Poon</name><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name></country><country name="États-Unis"><region name="État de New York"><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name></region><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><name sortKey="Li, Ye" sort="Li, Ye" uniqKey="Li Y" first="Ye" last="Li">Ye Li</name><name sortKey="Ma, Cuiqing" sort="Ma, Cuiqing" uniqKey="Ma C" first="Cuiqing" last="Ma">Cuiqing Ma</name><name sortKey="Wang, Lili" sort="Wang, Lili" uniqKey="Wang L" first="Lili" last="Wang">Lili Wang</name><name sortKey="Yu, Fei" sort="Yu, Fei" uniqKey="Yu F" first="Fei" last="Yu">Fei Yu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002064 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002064 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:3765664
|texte= A safe and convenient pseudovirus-based inhibition assay to detect neutralizing antibodies and screen for viral entry inhibitors against the novel human coronavirus MERS-CoV
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23978242" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |